Fwd: Re: [Histonet] MMA sections

[Melanie Black]

Hi Marseille

This works very well, you need to de-resin in Xylene at 37 grees. Two 
changes for 15 minutes each. Then go on with normal wax staining 
process, i.e dehydrate, block primary etc, etc.

You can find papers written by Neil Hand on this subject. It is tried 
and tested with great results.

Good Luck
Melanie Black
Cardio Vascular Research
University of Cape Town
South Africa.



>Date: Thu, 9 Oct 2003 08:16:20 +0200
>To: Myri37 <@t> aol.com
>From: Melanie Black <ctsblack <@t> capeheart.uct.ac.za>
>Subject: Re: [Histonet] MMA sections
>Cc:
>Bcc:
>X-Attachments:
>
>>Hello everyone
>>i need to do ummunostaining on MMA sections of 5 Um deplasticised 
>>in xylene, i tried one time with a protocol used for paraffin 
>>section, but it doesn't work, i deplasiticised MMA in aceton 2 X 10 
>>minutes at 56 °C
>>Please, do you have a protocol especially for MMA sections, or 
>>special embedding in MMA for immunostaning, or any advice for 
>>deplasticise..
>>thank you very much in advance
>>Myriam Baali
>>
>>Natural Implant
>>Marseille
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031009/5e762178/attachment.htm