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sections</title></head><body>
<div>Hi Marseille</div>
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<div>This works very well, you need to de-resin in Xylene at 37 grees.
Two changes for 15 minutes each. Then go on with normal wax staining
process, i.e dehydrate, block primary etc, etc.</div>
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<div>You can find papers written by Neil Hand on this subject. It is
tried and tested with great results.</div>
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<div>Good Luck</div>
<div>Melanie Black</div>
<div>Cardio Vascular Research</div>
<div>University of Cape Town</div>
<div>South Africa.</div>
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<blockquote type="cite" cite>Date: Thu, 9 Oct 2003 08:16:20 +0200<br>
To: Myri37@aol.com<br>
From: Melanie Black <ctsblack@capeheart.uct.ac.za><br>
Subject: Re: [Histonet] MMA sections<br>
Cc:<br>
Bcc:<br>
X-Attachments:<br>
<blockquote type="cite" cite><font face="Arial" size="-1">Hello
everyone<br>
i need to do ummunostaining on MMA sections of 5 Um deplasticised in
xylene, i tried one time with a protocol used for paraffin section,
but it doesn't work, i deplasiticised MMA in aceton 2 X 10 minutes at
56 °C<br>
Please, do you have a protocol especially for MMA sections, or special
embedding in MMA for immunostaning, or any advice for
deplasticise..<br>
thank you very much in advance<br>
Myriam Baali<br>
<br>
Natural Implant<br>
Marseille</font></blockquote>
</blockquote>
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