[Histonet] mast cells

[Nick Kirk]

We use a simple 1% aqueous Toluidine blue technique. Stain in the solution
for about 10-15 minutes, blot dry and mount.
Mast cell granules purple, rest of cells/tissue blue.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England


   -----Original Message-----
  From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Noreen Gilman
  Sent: 01 October 2003 20:00
  To: histonet <@t> lists.utsouthwestern.edu
  Subject: [Histonet] mast cells


  Can anyone recommend a stain for mast cells? The one we do just doesn't
seem to be working as well as we'd like it to.
  thanks
  noreen

  Noreen Gilman, BS, HT (ASCP) QIHC
  Histopathology Supervisor
  Broward General Medical Center
  Ft. Lauderdale, FL 33316
  954.355.4358 Phone
  954.355.4139 Fax
  954.387.0213 Pager

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