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<DIV><FONT face=Arial color=#0000ff><SPAN class=828504920-01102003>We use a
simple 1% aqueous Toluidine blue technique. Stain in the solution for about
10-15 minutes, blot dry and mount.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff><SPAN class=828504920-01102003>Mast cell
granules purple, rest of cells/tissue blue.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff><SPAN
class=828504920-01102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff><SPAN class=828504920-01102003>Nick
Kirk</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff><SPAN
class=828504920-01102003>Histopathology</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff><SPAN
class=828504920-01102003>Hinchingbrooke Hospital</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff><SPAN
class=828504920-01102003>Huntingdon</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff><SPAN
class=828504920-01102003>England</SPAN></FONT></DIV>
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<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma><SPAN
class=828504920-01102003> </SPAN>-----Original
Message-----<BR><B>From:</B> histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]<B>On Behalf Of </B>Noreen
Gilman<BR><B>Sent:</B> 01 October 2003 20:00<BR><B>To:</B>
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> [Histonet] mast
cells<BR><BR></DIV></FONT>
<DIV>Can anyone recommend a stain for mast cells? The one we do just doesn't
seem to be working as well as we'd like it to.</DIV>
<DIV>thanks</DIV>
<DIV>noreen</DIV>
<DIV> </DIV>
<DIV>Noreen Gilman, BS, HT (ASCP) QIHC<BR>Histopathology Supervisor<BR>Broward
General Medical Center<BR>Ft. Lauderdale, FL 33316<BR>954.355.4358
Phone<BR>954.355.4139 Fax<BR>954.387.0213 Pager</DIV>
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