[Histonet] Whole bee embryo fixation

[Geoff McAuliffe]

Check out "Phase partition fixation and staining of Drosophila eggs" by 
Zalokar and Erb, Stain Technology 52:89, 1977.

Caroline Stott wrote:

> Hello,
> Has anyone had any experience with whole bee embryo fixation?  We can 
> bleach off the outer membrane which is no problem.  However we are 
> having trouble with fixatives penetrating the inner membrane.    If we 
> make a hole in it, the embryo explodes.  Can anyone help?
> Thanks
> Caroline
>
> Caroline Stott
>
> Histology Service Unit
> Medical School
> University of Otago
> Dunedin
> (03) 479 7152
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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