[Histonet] separated cells continued
Bernadette Weston
bernaweston <@t> hotmail.com
Fri Nov 21 13:28:13 CST 2003
Hope this helps my problem:
Here is our processing program:
Formalin 1:00
Formalin 2:30
70% ETOH :50
80% ETOH :50
95% ETOH :50
95% ETOH :50
100% ETOH :50
100% ETOH :50
Paraffin 1:00
Paraffin 1:00
Paraffin 1:00
The paraffin station are at 58 degrees the other stations are ambient the
pressure is on all stations.
The sections do not appear to be improperly processed when cutting, we use
accuride blades, we get a nice ribbon and the sections lay out in the
waterbath, we cut at 3 and 4 microns. We put the slides in a 60 degree
oven.
pictures @ histonet images
Bernadette Weston HT
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