[Histonet] different types of processors

[Charles W. Scouten, Ph.D.]

There are many real advantages to a dip and move system (OPEN)  when
compared to a sealed flush (CLOSED) system. The following points
highlight the operational differences 1) Time - the minimum cycle time
for a flush system is 2 hours. This is due to the heating phase for the
paraffin and the additional rinse cycles. For quick procedures such as
biopsies, 2 hours is an inconvenient cycle time when it can be done in
30 minutes with a TPC system. A procedure that would take only 8 hours
with the TPC will take 12 hours in a sealed system. 2) Artifacts - In
order to introduce the paraffin in a sealed system, the chamber must be
heated. This heating process can cause dead spots, shadows, or artifacts
in your tissue. The TPC system does not create such artifacts. 3)
Maintenance - The TPC system does not rely on pumps to complete the
protocols. The sealed system requires pumps which can fail. These can be
costly and inconvenient to replace. 4) Contamination - Although the
sealed systems are thought to be contamination free, they do require
cleaning after every protocol. Proteins are routinely washed out of
sections during processing. These proteins will settle in the bottom of
flush storage tanks and will be present for future protocols. In order
to remove all contaminates, the system must be cleaned. 5) Cost - The
TPC system uses a much lower volume of reagents and chemicals. These add
up over the coarse of a year. Have you actually calculated your annual
costs and looked at the potential savings? 6) Tissue Shrinking - The TPC
system does not expose the tissue to a heating cycle and therefore does
not cause the tissue to shrink.

 

I am also attaching a detailed data sheet here. If you have any
questions or would like to discuss this further please feel free to
contact me anytime. 

 

Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com <mailto:cwscouten <@t> myneruolab.com>  
www.myneurolab.com 

 

-----Original Message-----
From: JCarpenter764 <@t> aol.com [mailto:JCarpenter764 <@t> aol.com] 
Sent: Thursday, November 20, 2003 3:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] different types of processors

 

Would someone please explain the difference between an open processor
and a closed processor....

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