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<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'> </span></font></p>
<p class=MsoNormal><span class=product><font size=3 face=Verdana><span
style='font-size:12.0pt;font-family:Verdana'>There are many real advantages to
a dip and move system (OPEN) when compared to a sealed flush (CLOSED)
system. The following points highlight the operational differences 1) Time -
the minimum cycle time for a flush system is 2 hours. This is due to the
heating phase for the paraffin and the additional rinse cycles. For quick
procedures such as </span></font></span><span class=product><font face=Verdana><span
style='font-family:Verdana'>bio</span></font></span><span class=product><font
face=Verdana><span style='font-family:Verdana'>psies, 2 hours is an
inconvenient cycle time when it can be done in 30 minutes with a TPC system. A
procedure that would take only 8 hours with the TPC will take 12 hours in a
sealed system. 2) Artifacts – In order to introduce the paraffin in a
sealed system, the chamber must be heated. This heating process can cause dead
spots, shadows, or artifacts in your tissue. The TPC system does not create
such artifacts. 3) Maintenance – The TPC system does not rely on pumps to
complete the protocols. The sealed system requires pumps which can fail. These
can be costly and inconvenient to replace. 4) Contamination – Although
the sealed systems are thought to be contamination free, they do require
cleaning after every protocol. Proteins are routinely washed out of sections
during processing. These proteins will settle in the bottom of flush storage
tanks and will be present for future protocols. In order to remove all
contaminates, the system must be cleaned. 5) Cost – The TPC system uses a
much lower volume of reagents and chemicals. These add up over the coarse of a
year. Have you actually calculated your annual costs and looked at the
potential savings? 6) Tissue Shrinking – The TPC system does not expose
the tissue to a heating cycle and therefore does not cause the tissue to shrink.</span></font></span></p>
<p class=MsoNormal><span class=product><font size=3 face=Verdana><span
style='font-size:12.0pt;font-family:Verdana'> </span></font></span></p>
<p class=MsoNormal><span class=product><font size=3 face=Verdana><span
style='font-size:12.0pt;font-family:Verdana'>I am also attaching a detailed
data sheet here. If you have any questions or would like to discuss this
further please feel free to contact me anytime. </span></font></span></p>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'> </span></font></p>
<div>
<p><font size=3 color=navy face="Comic Sans MS"><span style='font-size:12.0pt;
font-family:"Comic Sans MS";color:navy'>Charles W. Scouten, Ph.D.</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><b><font color="#339966"><span style='color:#339966;font-weight:
bold'>myNeuroLab.com</span></font></b><font color=navy><span style='color:navy'>
<br>
</span></font><font size=2 color=navy face="Bookman Old Style"><span
style='font-size:10.0pt;font-family:"Bookman Old Style";color:navy'>5918
Evergreen Blvd.</span></font><font color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face="Bookman Old Style"><span
style='font-size:10.0pt;font-family:"Bookman Old Style";color:navy'>St. Louis</span></font><font
size=2 color=navy face="Bookman Old Style"><span style='font-size:10.0pt;
font-family:"Bookman Old Style";color:navy'>, </span></font><font size=2
color=navy face="Bookman Old Style"><span style='font-size:10.0pt;font-family:
"Bookman Old Style";color:navy'>MO</span></font><font size=2 color=navy
face="Bookman Old Style"><span style='font-size:10.0pt;font-family:"Bookman Old Style";
color:navy'> </span></font><font size=2 color=navy face="Bookman Old Style"><span
style='font-size:10.0pt;font-family:"Bookman Old Style";color:navy'>63134</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face="Bookman Old Style"><span
style='font-size:10.0pt;font-family:"Bookman Old Style";color:navy'>Ph: 314 522
0300 </span></font><font color=navy><span style='color:navy'><br>
</span></font><font size=2 color=navy face="Bookman Old Style"><span
style='font-size:10.0pt;font-family:"Bookman Old Style";color:navy'>FAX
314 522 0377</span></font><font color=navy><span style='color:navy'> <br>
<a href="mailto:cwscouten@myneruolab.com">cwscouten@myneurolab.com</a> <br>
www.myneurolab.com </span></font></p>
</div>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'> </span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Original Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> JCarpenter764@aol.com
[mailto:JCarpenter764@aol.com] <br>
<b><span style='font-weight:bold'>Sent:</span></b> </span></font><font size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>Thursday,
November 20, 2003</span></font><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'> </span></font><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>3:10 PM</span></font><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'><br>
<b><span style='font-weight:bold'>To:</span></b> histonet@lists.utsouthwestern.edu<br>
<b><span style='font-weight:bold'>Subject:</span></b> [Histonet] different
types of processors</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Would someone please explain the
difference between an open processor and a closed processor....</span></font></p>
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