[Histonet] Anti-beta-galactosidase antibodies

[Sharon Cooperman]

Hi.  Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed 
paraffin embedded tissue.  We used it on a transgenic mouse 
expressing Beta gal - pretty weak but detectable with ABC kit and 
HRP.  I think most of the transgenics express weakly and I would 
consider using tyramide (TSA) if you need more signal because 
background is very low.  I also think some of the Abcam anti-beta gal 
abs are pretty good (I don't know which ones, I borrowed them).

Sharon

>Has anyone had any experience with polyclonal antibody against
>b-galactosidase? What reporter system have you used? Thank you.
>Tom Kuzniar
>
>
>
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-- 
Sharon Cooperman       	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-7735
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