[Histonet] thin sectioning of PMMA

[John Baker]

Howdy all, Does anyone have a good protocol for getting thin (4-5 
micron) sections from PMMA embedded bone? We use a Riechert-Jung 
Polycut with tungsten knives for sectioning.  A problem in the past 
has been getting the sections to stick to a slide with curling and 
wrinkles being the cause.  Fluorescence of the sections will be 
looked at in future studies.  Thanks for any assistance in this 
matter!  John
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Today is the Tomorrow you worried about Yesterday. Was
it worth it?

Birthdays are good for you. Statistics show that the
people who have the most live the longest.

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John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635