[Histonet] chondrotin sulfate and hyaluronic acid in decalcifed bone sections

[Elizabeth Chlipala]

Hello All
 
Is it possible to stain specifically for chondrotin sulfate or
hyaluronic acid in the cartilage of decalcified knee joints (guinea
pig).  I know that Alcian Blue ph 2.5 will stain for hyaluronic acid.  I
do have a previous post from John Kiernan that explains a pretreatment
process for the Alcian Blue pH 2.5 to make it specific for hyaluronic
acid. But I was wondering if anyone had any other suggestions and what I
could do for staining of chondrotin sulfate.
 
Thanks in advance.
 
Liz 
 
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
 
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