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<div class=Section1>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Hello All<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Is it possible to stain specifically for <span class=SpellE>chondrotin</span>
sulfate or <span class=SpellE>hyaluronic</span> acid in the cartilage of
decalcified knee joints (guinea pig)<span class=GramE>.</span> <span
style='mso-spacerun:yes'> </span>I know that <span class=SpellE>Alcian</span>
Blue ph 2.5 will stain for <span class=SpellE>hyaluronic</span> acid.<span
style='mso-spacerun:yes'> </span>I do have a previous post from John <span
class=SpellE>Kiernan</span> that explains a pretreatment process for the <span
class=SpellE>Alcian</span> Blue pH 2.5 to make it specific for <span
class=SpellE>hyaluronic</span> acid. But I was wondering if anyone had any
other suggestions and what I could do for staining of <span class=SpellE>chondrotin</span>
sulfate.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Thanks in advance.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Liz <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>Elizabeth A. Chlipala, BS, HTL(ASCP)</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>Premier Histology Laboratory, LLC</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><st1:address><st1:Street><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>P.O. Box</span></font></st1:Street><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial;mso-no-proof:
yes'> 18592</span></font></st1:address><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'> </span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><st1:place><st1:City><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>Boulder</span></font></st1:City><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial;mso-no-proof:
yes'>, </span></font><st1:State><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>Colorado</span></font></st1:State><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial;mso-no-proof:
yes'> </span></font><st1:PostalCode><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>80308</span></font></st1:PostalCode></st1:place><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>Office: (303) 735-5001</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>Fax: (303) 735-3540</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><st1:PersonName><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>lizchlipala@premierhistology.com</span></font></st1:PersonName><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>www.premierhistology.com</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=3 face="Times New Roman"><span style='font-size:
12.0pt;mso-no-proof:yes'><o:p> </o:p></span></font></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>Ship to Address:</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>Premier Histology Laboratory</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><st1:place><st1:PlaceType><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>University</span></font></st1:PlaceType><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial;mso-no-proof:
yes'> of </span></font><st1:PlaceName><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>Colorado</span></font></st1:PlaceName></st1:place><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;mso-no-proof:yes'>MCBD, Room A3B40</span></font><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><st1:place><st1:City><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>Boulder</span></font></st1:City><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial;mso-no-proof:
yes'>, </span></font><st1:State><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>Colorado</span></font></st1:State><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial;mso-no-proof:
yes'> </span></font><st1:PostalCode><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;mso-no-proof:yes'>80309</span></font></st1:PostalCode></st1:place><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoAutoSig><font size=3 face="Times New Roman"><span style='font-size:
12.0pt;mso-no-proof:yes'> <o:p></o:p></span></font></p>
<p class=MsoAutoSig><b><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;font-weight:bold;mso-no-proof:yes'>_________________________________</span></font></b><span
style='mso-no-proof:yes'> <br>
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