[Histonet] heart IHC work

[Patsy Ruegg]

I will definitely make frozen sections from the frozen heart but I was just
wondering what would be a good way to then process some of it into paraffin
for IHC.  I used to do this with the frozen OCT block by just placing the
block into cold PBS and then eventually into RT fixative after a while, is
this a proper method to preserve antigens?
Patsy

-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg
Sent: Tuesday, November 11, 2003 2:14 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] heart IHC work

Friends,
I am involved in a project that requires doing IHC for Lamin A/C on heart
tissue.  If anyone has experience with this please tell me all you know.
Also, since most of this is DNA work they have pieces of fresh heart frozen
at -70 dC.  What would be the best way to fix and process the frozen heart
so that it would be well preserved for IHC.
Thank you for advise in this matter
Patsy


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