[Histonet] heart IHC work

[Gayle Callis]

Cut frozen sections, then fix for IHC.  Why compromise the tissue twice,
frozen then thaw, fix and process?  That is how I interpret this message,
correct? 



At 02:13 PM 11/11/2003 -0700, you wrote:
>Friends,
>I am involved in a project that requires doing IHC for Lamin A/C on heart
>tissue.  If anyone has experience with this please tell me all you know.
>Also, since most of this is DNA work they have pieces of fresh heart frozen
>at -70 dC.  What would be the best way to fix and process the frozen heart
>so that it would be well preserved for IHC.
>Thank you for advise in this matter
>Patsy
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)