[Histonet] Amyloid Precursor Protein

[Marion Hiles]

We use ZYMED BAPP Cat No. 13-0200 at 1:3000 overnight after microwaving in
citrate buffer pH6.

Bob Quilty
Dept of Neuropathology
Frenchay Hospital
Bristol UK

-----Original Message-----
From: Dana Settembre [mailto:settembr <@t> umdnj.edu]
Sent: 10 November 2003 16:39
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Amyloid Precursor Protein


Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human
tissue?
I have NovoCastra's monoclonal antibody.  I have used Alzheimer's
disease brain tissue that works with other antibodies.  I use a very
good Target Retreival solution from DakoCytomation in a steamer for 40
minutes.  I have used it at the recommended dilutions and I have used
them more potent than that too.  Our neuropathologist tells me that it
has been in use regularly for some time now.
HELP!
Dana Settembre
Immunohistochemistry Lab
Unversity Hospital-UMDNJ
Newark, NJ
USA

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