[Histonet] Amyloid Precursor Protein

[Drew Sally A.]

We are using Chemicon's cat#MAB348 at a 1:200 after  pressure cooker HIER in EDTA buffer...
This is run on our Ventana stainers for only an 8 min. titration.

Sally Ann Drew-MT(ASCP)
Univ. of Wisconsin Hospital & Clinics
IHC/ISH Laboratory 
600 Highland Ave. VAH-DM223
Madison, WI 53792-2472
608-265-6596
sa.drew <@t> hosp.wisc.edu


-----Original Message-----
From: Dana Settembre [mailto:settembr <@t> umdnj.edu]
Sent: Monday, November 10, 2003 10:39 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Amyloid Precursor Protein


Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human
tissue?
I have NovoCastra's monoclonal antibody.  I have used Alzheimer's
disease brain tissue that works with other antibodies.  I use a very
good Target Retreival solution from DakoCytomation in a steamer for 40
minutes.  I have used it at the recommended dilutions and I have used
them more potent than that too.  Our neuropathologist tells me that it
has been in use regularly for some time now.
HELP!
Dana Settembre
Immunohistochemistry Lab
Unversity Hospital-UMDNJ
Newark, NJ
USA

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