[Histonet] Amyloid Precursor Protein

[Richard Cartun]

We use Chemicon's MAB348 at 1:5,000 (overnight incubation) with
DakoCytomation's EnVision+/DAB+ detection.  Retrieval is performed in a
96 degree waterbath for 40 minutes.

Richard Cartun

>>> Dana Settembre <settembr <@t> umdnj.edu> 11/10/03 11:38AM >>>
Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human
tissue?
I have NovoCastra's monoclonal antibody.  I have used Alzheimer's
disease brain tissue that works with other antibodies.  I use a very
good Target Retreival solution from DakoCytomation in a steamer for 40
minutes.  I have used it at the recommended dilutions and I have used
them more potent than that too.  Our neuropathologist tells me that it
has been in use regularly for some time now.
HELP!
Dana Settembre
Immunohistochemistry Lab
Unversity Hospital-UMDNJ
Newark, NJ
USA

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