[Histonet] URGENT! Questions regarding snap freezing in liquid nitrogen with isopentene

[Geoff McAuliffe]

I learned to snap freeze with 2-methylbutane (isopentane) cooled by 
liquid nitrogen over 25 years ago and it was a common proceedure then. 
Try textbooks on histological technique for the original references.

Geoff

khor0011 <@t> flinders.edu.au wrote:

>Hi everyone,
>
>I am an Honours student, currently finishing up my Honours thesis. 
>I am wondering if anyone could give me some REFERENCES on snap freezing mouse 
>tissues using liquid nitrogen and ISOPENTENE. I have used liquid nitrogen ONLY 
>for snap freezing my mouse tissues in my project, but not with isopentene. And 
>i have been having cryostat sectioned mouse tissues that have a lot of tissue 
>degeneration, as well as artefacts after fixing in 2% para & 0.5% Glu, and i 
>got the idea from someone that this maybe due to the fact that i did not use 
>isopentene in the snap freezing process.
>
>So what i want is to discuss this in my thesis. So it would be great if 
>someone could give me any scientific references/comments/advice on this method 
>whereby isopentene is used in the snap freezing process, and why using this is 
>important. 
>
>I have been doing some internet searching myself, but any advice or help from 
>all histo experts out there would be greatly appreciated.
>
>Best regards,
>
>Hong Yuan Khor
>Flinders University
>South Australia
>
>
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>  
>

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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
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mcauliff <@t> umdnj.edu
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