[Histonet] URGENT! Questions regarding snap freezing in liquid nitrogen with isopentene

[John Kiernan]

There is abundant advice for you in
textbooks written 40 years ago (and
more recently too). ASK YOUR 
SUPERVISOR to suggest books or
review articles. 

For an honours-year project you should
be guided by your local boss and the
literature, not by answers from an
Internet listserver. Anyone can 
answer question. I could have answered
yours with all sorts of fake rubbish.
Would you have ueed it in your
honours dissertation?  
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
____________________________________________
khor0011 <@t> flinders.edu.au wrote:
> 
> Hi everyone,
> 
> I am an Honours student, currently finishing up my Honours thesis.
> I am wondering if anyone could give me some REFERENCES on snap freezing mouse
> tissues using liquid nitrogen and ISOPENTENE. I have used liquid nitrogen ONLY
> for snap freezing my mouse tissues in my project, but not with isopentene. And
> i have been having cryostat sectioned mouse tissues that have a lot of tissue
> degeneration, as well as artefacts after fixing in 2% para & 0.5% Glu, and i
> got the idea from someone that this maybe due to the fact that i did not use
> isopentene in the snap freezing process.
> 
> So what i want is to discuss this in my thesis. So it would be great if
> someone could give me any scientific references/comments/advice on this method
> whereby isopentene is used in the snap freezing process, and why using this is
> important.
> 
> I have been doing some internet searching myself, but any advice or help from
> all histo experts out there would be greatly appreciated.
> 
> Best regards,
> 
> Hong Yuan Khor
> Flinders University
> South Australia
> 
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