[Histonet] Re: tape

Fri Nov 7 10:48:28 CST 2003

My $.02. I think there is a significant difference tape transfer for FROZEN
sections and PARAFFIN. I have found the paraffin system to be more
cumbersome and produce worse results than  careful sectioning. However the
system used for frozen sections can be extremely useful.

Cynthia Favara 
NIAID/NIH/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
406-363-9317 

-----Original Message-----
From: Sebree Linda A. [mailto:la.sebree <@t> hosp.wisc.edu]
Sent: Thursday, November 06, 2003 3:24 PM
To: Thom Jensen; murphy.linda <@t> mayo.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: tape

In all fairness I feel that I have to add my $.02 worth.  In my last
research position (a number of years ago) I needed to section cross sections
of frozen rabbit heart that had had radioactive elements perfused through
it.  We needed adjacent sections that would be as identical as possible in
order to overlay autoradiographic images and histochemically stained
images.  Using the Instrumedics tape transfer system, I was able to produce
exquisite, near identical sections that enabled us to compile the data we
needed for our research project.  Granted, I never tried the system with
paraffin sectioning but at least in sectioning frozens, the system worked
brilliantly for me. 

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: Thom Jensen [mailto:tissuearray <@t> hotmail.com]
Sent: Thursday, November 06, 2003 2:38 PM
To: murphy.linda <@t> mayo.edu; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: tape

Linda,

Thank you for your input.  Not a lot of people want to talk about the
problems with the Tape-Transfer System.  We also have two in our lab and I
have tried on several occasions to work with Instrumedics to understand how
it can be used.  Eventually they stopped emailing me which ended our
collaboration.  Now I don't recommend the tape method to anyone.  I have
published two articles on Array Instruction in The Journal of
Histotechnology and I thought at one time that the Tape Method could me a
good alternative for techs that have a problem cutting arrays.  But now I
tell them to find someone in their area that can cut well.  

Thanks again,

Thom Jensen

For more information on Tissue Microarray Instruction visit:
www.arrayworkshop.com <http://www.arrayworkshop.com/> 

>From: "Murphy, Linda M." 
>To: "'tissuearray <@t> hotmail.com'" 
>Subject: tape 
>Date: Thu, 6 Nov 2003 13:29:53 -0600 
> 
>Tom, 
>I am so excited that people are publicly voicing just how they feel about
this most horrendous tape method. First off, I have felt all along that it
is an insult to any HistoTech who cuts........the two technicians who do the
cutting in my lab cut absolutely gorgeous sections.......special attention
is paid to the TMA's and they get consistent beautiful sections with little
to no distortions. My lab director was so impressed, however, by the
articles she had read about the "success" of the tape method, we were asked
to buy one for each cutting station.....they now just sit in the drawer in
case a researcher feels the need. I have a feeling we will end up tossing
them in due time. I am all for the poll. I no longer participate in the
Histonet and was given this information from a friend. You are certainly
more than welcome to express my views if you like. I would though like to
state this is nothing against Bernice and Instrumedics. They have known for
quite some time that their tape method needs work. I would hope they would
benefit from the information they would receive from a poll such as you
would like to undertake. Please feel free to contact me at any
time.......thanks. Linda 
> 
>Linda M. Murphy, HT(ASCP) 
>Supervisor, TACMA Shared Resource 
>10-36 Guggenheim 
>Mayo Foundation 
>Rochester, MN 55905 
>Voice:(507)266-5115 
>Fax:(507)284-8105 
>email:murphy.linda <@t> mayo.edu 
> 

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