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<DIV><SPAN class=029324516-07112003><FONT face=Georgia>My $.02. I think there is
a significant difference tape transfer for FROZEN sections and PARAFFIN. I
have found the paraffin system to be more cumbersome and produce worse results
than careful sectioning. However the system used for frozen sections can
be extremely useful.</FONT></SPAN></DIV>
<DIV> </DIV>
<P><FONT face="BernhardFashion BT" color=#000080>Cynthia Favara</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>NIAID/NIH/RML/LPVD</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>903 South 4th Street</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>Hamilton, MT 59840</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>406-363-9317</FONT> </P>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Sebree Linda A.
[mailto:la.sebree@hosp.wisc.edu]<BR><B>Sent:</B> Thursday, November 06, 2003
3:24 PM<BR><B>To:</B> Thom Jensen; murphy.linda@mayo.edu;
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> RE: [Histonet] Re:
tape<BR><BR></FONT></DIV>
<DIV><SPAN class=450470222-06112003><FONT face=Arial color=#0000ff size=2>In
all fairness I feel that I have to add my $.02 worth. In my last
research position (a number of years ago) I needed to section cross
sections of frozen rabbit heart that had had radioactive elements perfused
through it. We needed adjacent sections that would be as identical
as possible in order to overlay autoradiographic images and histochemically
stained images. Using the Instrumedics tape transfer system,
I was able to produce exquisite, near identical sections that enabled us
to compile the data we needed for our research project. Granted, I
never tried the system with paraffin sectioning but at least in sectioning
frozens, the system worked brilliantly for me. </FONT></SPAN></DIV>
<P><FONT size=2>Linda A. Sebree, HT(ASCP)<BR>University of Wisconsin Hospital
& Clinics<BR>IHC/ISH Laboratory<BR>A4/204-2472<BR>600 Highland
Ave.<BR>Madison, WI 53792<BR>(608)265-6596<BR>FAX:
(608)262-7174<BR></FONT></P>
<BLOCKQUOTE>
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Thom Jensen
[mailto:tissuearray@hotmail.com]<BR><B>Sent:</B> Thursday, November 06, 2003
2:38 PM<BR><B>To:</B> murphy.linda@mayo.edu;
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> [Histonet] Re:
tape<BR><BR></FONT></DIV>
<DIV>
<DIV>
<DIV>
<P>Linda,</P></DIV>
<P>Thank you for your input. Not a lot of people want to talk about
the problems with the Tape-Transfer System. We also have two in our
lab and I have tried on several occasions to work with Instrumedics to
understand how it can be used. Eventually they stopped emailing me
which ended our collaboration. Now I don't recommend the tape method
to anyone. I have published two articles on Array Instruction in The
Journal of Histotechnology and I thought at one time that the Tape Method
could me a good alternative for techs that have a problem cutting
arrays. But now I tell them to find someone in their area that
can cut well. </P></DIV>
<P>Thanks again,</P>
<DIV></DIV>
<P>Thom Jensen</P>
<DIV></DIV>
<P> </P>
<DIV></DIV>
<P>For more information on Tissue Microarray Instruction visit: <A
href="http://www.arrayworkshop.com/">www.arrayworkshop.com</A></P>
<DIV></DIV>
<P> </P>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>From: "Murphy, Linda M." <MURPHY.LINDA@MAYO.EDU>
<DIV></DIV>
<DIV></DIV>>To: "'tissuearray@hotmail.com'" <TISSUEARRAY@HOTMAIL.COM>
<DIV></DIV>
<DIV></DIV>>Subject: tape
<DIV></DIV>
<DIV></DIV>>Date: Thu, 6 Nov 2003 13:29:53 -0600
<DIV></DIV>
<DIV></DIV>>
<DIV></DIV>
<DIV></DIV>>Tom,
<DIV></DIV>
<DIV></DIV>>I am so excited that people are publicly voicing just how
they feel about this most horrendous tape method. First off, I have felt all
along that it is an insult to any HistoTech who cuts........the two
technicians who do the cutting in my lab cut absolutely gorgeous
sections.......special attention is paid to the TMA's and they get
consistent beautiful sections with little to no distortions. My lab director
was so impressed, however, by the articles she had read about the "success"
of the tape method, we were asked to buy one for each cutting
station.....they now just sit in the drawer in case a researcher feels the
need. I have a feeling we will end up tossing them in due time. I am all for
the poll. I no longer participate in the Histonet and was given this
information from a friend. You are certainly more than welcome to express my
views if you like. I would though like to state this is nothing against
Bernice and Instrumedics. They have known for quite some time that their
tape method needs work. I would hope they would benefit from the information
they would receive from a poll such as you would like to undertake. Please
feel free to contact me at any time.......thanks. Linda
<DIV></DIV>
<DIV></DIV>>
<DIV></DIV>
<DIV></DIV>>Linda M. Murphy, HT(ASCP)
<DIV></DIV>
<DIV></DIV>>Supervisor, TACMA Shared Resource
<DIV></DIV>
<DIV></DIV>>10-36 Guggenheim
<DIV></DIV>
<DIV></DIV>>Mayo Foundation
<DIV></DIV>
<DIV></DIV>>Rochester, MN 55905
<DIV></DIV>
<DIV></DIV>>Voice:(507)266-5115
<DIV></DIV>
<DIV></DIV>>Fax:(507)284-8105
<DIV></DIV>
<DIV></DIV>>email:murphy.linda@mayo.edu
<DIV></DIV>
<DIV></DIV>>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV></DIV><BR clear=all>
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