Fwd: [Histonet] cytokeratin

[Ian Montgomery]

Isabelle,
         Used Cytokeratin 8 from Dako after Trysin on mouse tissue followed 
by Dako Ark. Beautiful results.
Ian.


>From: "Lombaert" <i.lombaert <@t> med.rug.nl>
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>Dear Histonetters,
>
>Has anyone tried to stain cytokeratins (7, 8.12, 14 and 18 from
>Sigma) on mouse/murine salivary gland tissues? I already tried 10
>mM sodium citrate pH 6.0 for 30 min in the microwave,
>
>as well as 0.1M TrisHCl pH 9.5,
>no target retrieval at all,
>proteinase K for 5 min (20 mg/ml)
>
>but no results. I only could detect something with the enzym
>proteinase K, although my tissue was disrupted badly.
>My sections are paraffine imbedded and 4% formaldehyde fixed.
>
>
>Kind regards,
>Isabelle
>
>
>-----------------------
>Ir. Isabelle Lombaert, MSc
>PhD student
>University of Groningen
>Faculty of Medical Sciences
>Antonius Deusinglaan 1
>Building 3215, 5th floor, Room 553
>9713 AV Groningen
>The Netherlands
>e-mail: I.Lombaert <@t> med.rug.nl
>Tel.: +31 (0)50 363 29 15
>
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Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk 
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