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<font size=3>Isabelle,<br>
<x-tab> </x-tab>Used
Cytokeratin 8 from Dako after Trysin on mouse tissue followed by Dako
Ark. Beautiful results.<br>
Ian.<br><br>
<br>
<blockquote type=cite class=cite cite>From: "Lombaert"
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Subject: [Histonet] cytokeratin<br>
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Dear Histonetters,<br><br>
Has anyone tried to stain cytokeratins (7, 8.12, 14 and 18 from <br>
Sigma) on mouse/murine salivary gland tissues? I already tried 10 <br>
mM sodium citrate pH 6.0 for 30 min in the microwave, <br><br>
as well as 0.1M TrisHCl pH 9.5,<br>
no target retrieval at all,<br>
proteinase K for 5 min (20 mg/ml)<br><br>
but no results. I only could detect something with the enzym <br>
proteinase K, although my tissue was disrupted badly. <br>
My sections are paraffine imbedded and 4% formaldehyde fixed. <br><br>
<br>
Kind regards,<br>
Isabelle<br><br>
<br>
-----------------------<br>
Ir. Isabelle Lombaert, MSc<br>
PhD student<br>
University of Groningen<br>
Faculty of Medical Sciences<br>
Antonius Deusinglaan 1<br>
Building 3215, 5th floor, Room 553<br>
9713 AV Groningen<br>
The Netherlands<br>
e-mail: I.Lombaert@med.rug.nl<br>
Tel.: +31 (0)50 363 29 15<br><br>
_______________________________________________<br>
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<x-sigsep><p></x-sigsep>
<font size=3 color="#0000FF">Dr. Ian Montgomery,<br>
Histotechnology,<br>
Graham Kerr Building,<br>
Institute of Biomedical & Life Sciences,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855 <br>
Office: 4652<br>
Lab: 6644.<br>
Pager: 07625 702883<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></body>
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