[Histonet] Genetics anyone?

[Philip Oshel]

Cheryl,

Periodic acid-Schiff's stains nucleic acids stochiometrically, 
allowing ploidy determination by microspectrophotometry. Botanists 
used to do this in the 60s and 70s before the molecular types killed 
microscopy. The light 'scope I learned this one was an early 60s 
vintage, including the spec. Check around the botanists at LSU -- the 
older ones -- and see if any of them remember this. Ploidy is 
particularly important in botany.

Phil

>Good morning - This questions was put to me yesterday and, knowing
>little about genetics, I'm stumped.  Can any of you help me.  This
>researcher is collecting samples which he says are either diploid,
>triploid or tetraploid.  He states that someone told him there is a
>stain techniques that "would distinguish the 'ploidy' by the intensity
>of the stained tissue".
>
>Have any of you heard of such a thing or know someone I can contact for
>this "unusual" question?  Thank you, in advance.  Cheryl
>
>
>Cheryl Crowder, BA, HTL(ASCP)
>Chief Technologist
>Anatomic Pathology
>Department of Pathobiological Sciences
>School of Veterinary Medicine
>Louisiana State University
>Skip Bertman Drive
>Baton Rouge, LA  70803
>
>225-578-9734
>FAX:  225-578-9720
>
>
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-- 
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)