[Histonet] How to educe back ground

[Baowei Peng]

Hi,all
Thanks for your kindly responses.
My second Ab is said to be a mAb in the description file from=
 manufacture. But do not say if it was absorbed by rat IgG,=
 problely not.
David Wright is right. There is an inflammatory reaction as seen=
 by mononuclear cell infiltration and diapedesis in the tissue.
So maybe add rat serum to blocking and antibody diluent reagent=
 will work. I will try this out. 

=09=09=09=09

=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1Baowei Peng
=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1pengbaowei <@t> 21cn.com
=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A12003-12-30