[Histonet] How to reduce background stain?

Baowei Peng pengbaowei <@t> 21cn.com
Fri Dec 26 02:41:36 CST 2003

I'm doing IHC on rat skeletal muscle. I got a heavy background=
 stain on all sections and control section without 1st AB. But=
 there is no stain on the control section without 2nd Ab.
It looks like the 2nd Ab will react with the rat tissue. My first=
 Ab is derived from Mouse, and second Ab is anti-mouse.
What could I do to reduce the background stain?


=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1Baowei Peng
=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1pengbaowei <@t> 21cn.com

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