[Histonet] Re : Kappa and Lambda
TMcNemar <@t> lmhealth.org
Wed Dec 17 05:02:42 CST 2003
We use Dako's antibodies with a steamer. They are beautiful. We no longer
use the pressure cooker.
Tom Mc Nemar HT(ASCP)
Licking Memorial Hospital
From: Anissa Choi [mailto:anissachoi <@t> hotmail.com]
Sent: Tuesday, December 16, 2003 10:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re : Kappa and Lambda
Does anyone pressure cook the paraffin sections before immunostaining for
Kappa and Lambda?
Our regular protocols : no pretreatment for B5, Protease for buffered
formalin. I have found that
there are a lot more plasma cells get stained after pressure cooking but
backround is heavy though.
Thanks in advance for your input.
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