[Histonet] The best staining method for new bone formation
Barry R Rittman
Barry.R.Rittman <@t> uth.tmc.edu
Mon Dec 15 11:53:08 CST 2003
Hi - happy holidays.
I would recommend that you use a trichrome such as Masson's trichrome
when osteoid is stained green and bone red - this should allow easy
differentiation for image analysis.
Not sure if you are already doing this but it is customary to not only
count the thickness of osteoid but also the numbers of osteoblasts, bone
lining cells and osteoclasts per unit length.
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of Ronakorn
Sent: Monday, December 15, 2003 1:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] The best staining method for new bone
I would like to conduct the study to evaluate the action of Bone
Morphogenetic Protein (BMP) antagonist in prevent new bone formation.
But the well known H&E stain may be not good enough because both matrix
and new bone formation ( osteoid ) is also red, and it is also very hard
in quantitative evaluation by using computer program ,eg. NIH too.
Does anybody has some advice in better staining method or other
way in quantitative evaluation that able to differentiate the new bone
formation from the peripheral matrix.
Thank you very much for your answer in advance.
Graduate School of Biomedical Science
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