[Histonet] The best staining method for new bone formation

Barry R Rittman Barry.R.Rittman <@t> uth.tmc.edu
Mon Dec 15 11:53:08 CST 2003

Hi - happy holidays.
I would recommend that you use a trichrome such as Masson's trichrome
when osteoid is stained green and bone  red  -  this should allow easy
differentiation for image analysis.
Not sure if you are already doing this but it is customary to not only
count the thickness of osteoid but also the numbers of osteoblasts, bone
lining cells and osteoclasts per unit length.

	-----Original Message-----
	From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of Ronakorn
	Sent: Monday, December 15, 2003 1:14 AM
	To: histonet <@t> lists.utsouthwestern.edu
	Subject: [Histonet] The best staining method for new bone
	Hello everybody
	I would like to conduct the study to evaluate the action of Bone
Morphogenetic Protein (BMP) antagonist in prevent new bone formation.
But the well known H&E stain may be not good enough because both matrix
and new bone formation ( osteoid ) is also red, and it is also very hard
in quantitative evaluation by using computer program ,eg. NIH too. 
	Does anybody has some advice in better staining method or other
way in quantitative evaluation that able to differentiate the new bone
formation from the peripheral matrix. 
	Thank you very much for your answer in advance.
	Ronakorn Panjaphongse
	Graduate Student
	Graduate School of Biomedical Science
	Hiroshima University


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