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<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=066194817-15122003>Hi -
happy holidays.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=066194817-15122003>I
would recommend that you use a trichrome such as Masson's trichrome when osteoid
is stained green and bone red - this should allow easy
differentiation for image analysis.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=066194817-15122003>Not
sure if you are already doing this but it is customary to not only count the
thickness of osteoid but also the numbers of osteoblasts, bone lining cells and
osteoclasts per unit length.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=066194817-15122003>Barry</SPAN></FONT></DIV>
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<DIV></DIV>
<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] <B>On Behalf Of </B>Ronakorn
Panjaphongse<BR><B>Sent:</B> Monday, December 15, 2003 1:14 AM<BR><B>To:</B>
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> [Histonet] The best
staining method for new bone formation<BR><BR></FONT></DIV>
<DIV>Hello everybody</DIV>
<DIV> </DIV>
<DIV>I would like to conduct the study to evaluate the action of Bone
Morphogenetic Protein (BMP) antagonist in prevent new bone formation. But the
well known H&E stain may be not good enough because both matrix and new
bone formation ( osteoid ) is also red, and it is also very hard in
quantitative evaluation by using computer program ,eg. NIH too. </DIV>
<DIV> </DIV>
<DIV>Does anybody has some advice in better staining method or other
way in quantitative evaluation that able to differentiate the new bone
formation from the peripheral matrix. </DIV>
<DIV> </DIV>
<DIV>Thank you very much for your answer in advance.</DIV>
<DIV> </DIV>
<DIV>Ronakorn Panjaphongse</DIV>
<DIV>Graduate Student</DIV>
<DIV>Graduate School of Biomedical Science</DIV>
<DIV>Hiroshima University</DIV>
<DIV>Japan.</DIV>
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