[Histonet] Joey Wilkie (no subject)

[Caroline Stott]

Have you been filtering the haematoxylin before use?  Also, what 
haematoxylin are you using?  We use Harris haematoxylin and differentiate 
the slides in 0.5% acid/alcohol (conc HCL in 70% alc) for 10 
seconds.  These steps should eliminate any precipitate.
Caroline

At 09:30 13/12/03 +1300, you wrote:

>We are habing problems with brown precipitant on our slides after we have 
>stained with H&E on our automated stainer.  We do have a Tissue-Tek 
>coverslipper.  The pathologists say the the H&E staining is good.  We 
>called Sukura and they said that maybe our slides were not getting 
>completely dehydrated. We have tried uping the times on our stainer, we 
>are changing the alcohols almost after each rack and we are still getting 
>brown precipitant.  Our pathologist are saying that as they screen the 
>slides that they can see the brown precipitant forming.  When we coverslip 
>by hand we are not getting the brown precipitant.  We can use any and all 
>suggestions that we can get
>
>Thanks, Joey Wilkie, HT
>St. Mary's Hospital
>Grand Junction, Colorado
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Caroline Stott

Histology Service Unit
Medical School
University of Otago
Dunedin
(03) 479 7152 
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