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Have you been filtering the haematoxylin before use? Also, what
haematoxylin are you using? We use Harris haematoxylin and
differentiate the slides in 0.5% acid/alcohol (conc HCL in 70% alc) for
10 seconds. These steps should eliminate any precipitate.<br>
Caroline<br><br>
At 09:30 13/12/03 +1300, you wrote:<br><br>
<blockquote type=cite class=cite cite><font size=2>We are habing problems
with brown precipitant on our slides after we have stained with H&E
on our automated stainer. We do have a Tissue-Tek
coverslipper. The pathologists say the the H&E staining is
good. We called Sukura and they said that maybe our slides were not
getting completely dehydrated. We have tried uping the times on our
stainer, we are changing the alcohols almost after each rack and we are
still getting brown precipitant. Our pathologist are saying that as
they screen the slides that they can see the brown precipitant
forming. When we coverslip by hand we are not getting the brown
precipitant. We can use any and all suggestions that we can
get<br>
</font><br>
<font size=2>Thanks, Joey Wilkie, HT</font> <br>
<font size=2>St. Mary's Hospital</font> <br>
<font size=2>Grand Junction, Colorado</font> <br>
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<x-sigsep><p></x-sigsep>
Caroline Stott<br><br>
Histology Service Unit<br>
Medical School<br>
University of Otago<br>
Dunedin<br>
(03) 479 7152</font></body>
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