[Histonet] cacodylate vs PBS

Sharon Cooperman scoop <@t> mail.nih.gov
Fri Dec 12 16:49:38 CST 2003

Hi, histonetters.  There is a debate in my lab regarding which buffer 
is best for IHC on FFPE tissue.  I use 0.1M PBS made up the usual way 
(Sorensen's?)  Other people in my lab use NaCacodylate.  I have been 
told by various people that NaCacodylate is used for some EM purposes 
and doesn't preserve antigenicity or isn't the best choice for IHC 
for some other reason.  Does anyone have info or an opinion on this 
to either set me straight or provide me with ammunition for the next 
round of arguing?

Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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