[Histonet] (no subject)

[Laurie Colbert]

The coverslipping film is not completely adhering to the slides because:   1)  There's not enough xylene being dispensed onto the slides and the slides are drying out (it's better to dispense too much xylene than not enough)     2)  The white roller that helps "push" the film down onto the slide is not in the "down" position  3) The tissue is too thick.  We get this more often on decal slides than other slides.
 
Laurie Colbert
Pasadena, CA

-----Original Message-----
From: Joey Wilkie [mailto:JWilkie <@t> stmarygj.com]
Sent: Friday, December 12, 2003 12:31 PM
To: 'HistoNet <@t> pathology.swmed.edu'
Subject: [Histonet] (no subject)



We are habing problems with brown precipitant on our slides after we have stained with H&E on our automated stainer.  We do have a Tissue-Tek coverslipper.  The pathologists say the the H&E staining is good.  We called Sukura and they said that maybe our slides were not getting completely dehydrated. We have tried uping the times on our stainer, we are changing the alcohols almost after each rack and we are still getting brown precipitant.  Our pathologist are saying that as they screen the slides that they can see the brown precipitant forming.  When we coverslip by hand we are not getting the brown precipitant.  We can use any and all suggestions that we can get

Thanks, Joey Wilkie, HT 
St. Mary's Hospital 
Grand Junction, Colorado 
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