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<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=755334421-12122003>The
coverslipping film is not completely adhering to the slides because:
1) There's not enough xylene being dispensed onto the slides and the
slides are drying out (it's better to dispense too much xylene than not
enough) 2) The white roller that helps "push" the
film down onto the slide is not in the "down" position 3) The tissue is
too thick. We get this more often on decal slides than other
slides.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=755334421-12122003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=755334421-12122003>Laurie
Colbert</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=755334421-12122003>Pasadena, CA</SPAN></FONT></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Joey Wilkie
[mailto:JWilkie@stmarygj.com]<BR><B>Sent:</B> Friday, December 12, 2003 12:31
PM<BR><B>To:</B> 'HistoNet@pathology.swmed.edu'<BR><B>Subject:</B> [Histonet]
(no subject)<BR><BR></FONT></DIV>
<P><FONT face=Arial size=2>We are habing problems with brown precipitant on
our slides after we have stained with H&E on our automated stainer.
We do have a Tissue-Tek coverslipper. The pathologists say the the
H&E staining is good. We called Sukura and they said that maybe our
slides were not getting completely dehydrated. We have tried uping the times
on our stainer, we are changing the alcohols almost after each rack and we are
still getting brown precipitant. Our pathologist are saying that as they
screen the slides that they can see the brown precipitant forming. When
we coverslip by hand we are not getting the brown precipitant. We can
use any and all suggestions that we can get</FONT></P>
<P><FONT face=Arial size=2>Thanks, Joey Wilkie, HT</FONT> <BR><FONT face=Arial
size=2>St. Mary's Hospital</FONT> <BR><FONT face=Arial size=2>Grand Junction,
Colorado</FONT> <BR><FONT face=Arial size=2>This electronic message and all
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