[Histonet] (no subject)

[Fred Underwood]

Maybe you are not having enough xylene dispensed onto the slide during
coverslipping.  

Fred

>>> Joey Wilkie <JWilkie <@t> stmarygj.com> 12/12/03 03:30PM >>>
We are habing problems with brown precipitant on our slides after we
have
stained with H&E on our automated stainer.  We do have a Tissue-Tek
coverslipper.  The pathologists say the the H&E staining is good.  We
called
Sukura and they said that maybe our slides were not getting completely
dehydrated. We have tried uping the times on our stainer, we are
changing
the alcohols almost after each rack and we are still getting brown
precipitant.  Our pathologist are saying that as they screen the slides
that
they can see the brown precipitant forming.  When we coverslip by hand
we
are not getting the brown precipitant.  We can use any and all
suggestions
that we can get

Thanks, Joey Wilkie, HT
St. Mary's Hospital
Grand Junction, Colorado
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