[Histonet] archival fat storage
Barry R Rittman
Barry.R.Rittman <@t> uth.tmc.edu
Thu Dec 11 13:58:22 CST 2003
Although oil red O often survives archiving it doesn't always. Have you
considered seeing whether you can expose the tissue to osmium tetroxide
vapor, could then process to permanent mountant and have better
resolution? I see no reason that this would not work and might give you
a more permanent preparation.
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of Philip
Sent: Thursday, December 11, 2003 12:39 PM
To: Histonet <@t> Pathology.swmed.edu
Subject: [Histonet] archival fat storage
I have a person who has stained macrophages with Oil Red O for fat,
and now needs to make slides of them to be archived.
The cells are on plastic coverslips, and will be fixed with formalin.
Cultured cells, so no sectioning, etc. I have the usual references
for aqueous mounting media, but I'm wondering about the archival bit.
I have no problem, unfortunately, with archival storage of my
personal fat, but making aqueous fat mounts on slides that are
archival is a different matter. What is the best way to do this? (For
room temperature storage.)
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
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