[Histonet] blue d'alcian or whole PAS after ISH

Thu Dec 11 05:56:02 CST 2003

Help, I need a quick answer don't have time to try to figure things out by
myself and most of all only have a few slides so I don't want to lose the
results.

Will Alcian Blue staining alone at pH 4  or Alcian Blue +PAS destroy my
photographic emulsion coating? I used Alcian Bl + PAS technique long ago
and it was wonderful for identifying the different compartments of the GI
tract and identifying the cell types and which were secreting more. Now I
have an orphan receptor (all we know is it is a putative chloride channel
activated by chloride) that the mRNA is expressed in the secreting cells of
the GI tract but in a differential way, I want to see if the mRNA level is
a function of the level of secretion. or cell types  etc. So can I do a bl
Alcian / PAS or one or the other without destroying my emulsion and theryby
lose my signal??????????

Has  anyone tried to do either of these techniques after microscopic
photographic emulsion???? or if not can you suggest another technique???

faithfully yours,
Nancy Walker
Molecular Biology Scientist

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.walker <@t> sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001