[Histonet] BioCare Decloaker

[Favara, Cynthia (NIH/NIAID)]

Sue,
 
I have used this routinely for a little over a year. I do agree some of the
nuclear detail is less than wonderful but since we also have an H&E usually
cut serially it is not so problematic. I think the temperature range is not
a tight as I would like but I have found it to be very helpful. I usually
use citrate @pH 6 122C 20 minutes. Have used various buffers and even dilute
HCl and not had a problem with tissue destruction or sections falling off
slides.
 
c
 

Cynthia Favara 
NIAID/NIH/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
406-363-9317 

-----Original Message-----
From: STURROCK, Sue [mailto:Sue.STURROCK <@t> austin.org.au]
Sent: Wednesday, December 10, 2003 2:45 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] BioCare Decloaker




Hi Guys, 

We have recently purchased the BioCare Decloaker and have been impressed
with our improved antigen retrieval results.  Especially for antigens such
as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6.  However,
it has given us some problems with spiking of high temperatures and
subsequent destruction of tissue sections.  Also nuclear detail seems to be
reduced when using this instrument.

Has anyone else out there had problems like this and can we expect anything
else? 

Sue Sturrock 
Austin Health 
Melbourne, Australia 

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