[Histonet] BioCare Decloaker

Favara, Cynthia (NIH/NIAID) cfavara <@t> niaid.nih.gov
Wed Dec 10 17:29:02 CST 2003

I have used this routinely for a little over a year. I do agree some of the
nuclear detail is less than wonderful but since we also have an H&E usually
cut serially it is not so problematic. I think the temperature range is not
a tight as I would like but I have found it to be very helpful. I usually
use citrate @pH 6 122C 20 minutes. Have used various buffers and even dilute
HCl and not had a problem with tissue destruction or sections falling off

Cynthia Favara 
903 South 4th Street 
Hamilton, MT 59840 

-----Original Message-----
From: STURROCK, Sue [mailto:Sue.STURROCK <@t> austin.org.au]
Sent: Wednesday, December 10, 2003 2:45 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] BioCare Decloaker

Hi Guys, 

We have recently purchased the BioCare Decloaker and have been impressed
with our improved antigen retrieval results.  Especially for antigens such
as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6.  However,
it has given us some problems with spiking of high temperatures and
subsequent destruction of tissue sections.  Also nuclear detail seems to be
reduced when using this instrument.

Has anyone else out there had problems like this and can we expect anything

Sue Sturrock 
Austin Health 
Melbourne, Australia 


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