<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1">
<TITLE>BioCare Decloaker</TITLE>
<META content="MSHTML 5.50.4522.1800" name=GENERATOR></HEAD>
<BODY>
<DIV><SPAN class=818272223-10122003><FONT face=Georgia>Sue,</FONT></SPAN></DIV>
<DIV><SPAN class=818272223-10122003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=818272223-10122003><FONT face=Georgia>I have used this
routinely for a little over a year. I do agree some of the nuclear detail is
less than wonderful but since we also have an H&E usually cut serially it is
not so problematic. I think the temperature range is not a tight as I would like
but I have found it to be very helpful. I usually use citrate @pH 6 122C 20
minutes. Have used various buffers and even dilute HCl and not had a problem
with tissue destruction or sections falling off slides.</FONT></SPAN></DIV>
<DIV><SPAN class=818272223-10122003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=818272223-10122003><FONT face=Georgia>c</FONT></SPAN></DIV>
<DIV> </DIV>
<P><FONT face="BernhardFashion BT" color=#000080>Cynthia Favara</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>NIAID/NIH/RML/LPVD</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>903 South 4th Street</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>Hamilton, MT 59840</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>406-363-9317</FONT> </P>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> STURROCK, Sue
[mailto:Sue.STURROCK@austin.org.au]<BR><B>Sent:</B> Wednesday, December 10,
2003 2:45 PM<BR><B>To:</B>
'histonet@lists.utsouthwestern.edu'<BR><B>Subject:</B> [Histonet] BioCare
Decloaker<BR><BR></FONT></DIV><BR>
<P><FONT face=Arial size=2>Hi Guys,</FONT> </P>
<P><FONT face=Arial size=2>We have recently purchased the BioCare Decloaker
and have been impressed with our improved antigen retrieval results.
Especially for antigens such as Thyroid Transcription Factor 1 and Cytokeratin
cocktail 5 & 6. However, it has given us some problems with
spiking of high temperatures and subsequent destruction of tissue
sections. Also nuclear detail seems to be reduced when using this
instrument.</FONT></P>
<P><FONT face=Arial size=2>Has anyone else out there had problems like this
and can we expect anything else?</FONT> </P>
<P><FONT face=Arial size=2>Sue Sturrock</FONT> <BR><FONT face=Arial
size=2>Austin Health</FONT> <BR><FONT face=Arial size=2>Melbourne,
Australia</FONT> </P>
<P><FONT face=Arial
size=2>---------------------------------------------------------------------------------------------------------------------------------------------------------------</FONT></P>
<P><FONT face=Arial size=2>This email contains confidential information
intended only for the person named above and may be</FONT> <BR><FONT
face=Arial size=2>subject to legal privilege. If you are not the intended
recipient, any use, disclosure, copying or</FONT> <BR><FONT face=Arial
size=2>distribution of this transmission is prohibited. If you have received
this message in error, please notify</FONT> <BR><FONT face=Arial size=2>us
immediately by return email and delete the original email and any attachments.
Austin Health provides</FONT> <BR><FONT face=Arial size=2>no guarantee that
this transmission is free of virus or that it has not been intercepted or
altered.</FONT> </P>
<P><FONT face=Arial
size=2>---------------------------------------------------------------------------------------------------------------------------------------------------------------</FONT></P></BLOCKQUOTE></BODY></HTML>