[Histonet] SEM for collagen fibers

[Myri37 <@t> aol.com]

hello dear histonetters
first many thanks for your last precious help
I have few questions about identification of collagen fibers in resconstructed tissue
Do you think that fixation of small tissue with glutaraldéhyde for three days can avoid proper stainning of collagen fibers with trichrome stains, should i change the fixative to paraformaldéhyde for instance or reduce time of fixation ?
Do you know if it's possible to do SEM observations on sections which have been previously embedded in methylmetacrylate or paraffine ?
the issue is as well to observe collagen fibers and their orientation
i have no experience to doing that, does someone have a protocol ?
thank you so much for anyhelp


Myriam baali
Natural implant
163, av de luminy
marseille, france
tel: 04 91 80 41 89