[Histonet] RE: [IHCRG] IHC on cells on coverslips

[Tony Henwood]

Depending on the antibody. Most work after ethanol fixation, though some
require air-drying-cold acetone treatment.
You could attach a small paper clip to a corner of the coverslip to aid
manipulation.

Suggest fixation in 95% ethanol, 20min. Block endogenous enzyme (if
required), rinse in buffer.
You might consider marking the back of the coverslip with a marking pen at
this stage (the ink will dissolve in the dehydrating solutions). This will
aid in orientation.
Proceed with immunohistochemistry.
Remember to not let the coverslip dry out.
Mount Coverslip with a slide.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html


-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> colobio.com]
Sent: Wednesday, 10 December 2003 9:12 AM
To: Histonet <@t> Pathology. Swmed. Edu
Cc: Ihcrg <@t> Yahoogroups. Com
Subject: [IHCRG] IHC on cells on coverslips


Please advise how to manage IHC staining for cells grown on coverslips.
Patsy


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