[Histonet] Re: Stain for H.Pylori
bill501 <@t> mindspring.com
Mon Dec 8 13:28:58 CST 2003
I think the most important thing with the non-IHC syains is good
fixation of the mucin. We found that going from fomalin to Zn
formalin years ago made a big difference. Otherwise the mucin gets
stringy. and where strings cross can look like bacteria.
With a good specimen plus a good Giemsa, I can do most antrum bxs
with high dry. An occasional case requires oil, esp if there are a
lot of other bacteria present coming from the oropharynx or if the
preparation is suboptimal. I like the Alcian yellow better, but it is
considerably more expensive for us to do.
Bill Blank, MD
Heartland Lab, Inc
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