[Histonet] Re: Stain for H.Pylori

RSRICHMOND <@t> aol.com RSRICHMOND <@t> aol.com
Sun Dec 7 12:33:02 CST 2003

Lee and Peggy Wenk offer a Diff-Quik technique for Helicobacter pylori.

My personal preference - supported by at least some of the literature - is to 
omit the Diff-Quik I (eosin). - I've seen most of the stains in current use, 
in the various labs I work in. I think that IHC makes it easier to work 
without oil immersion. My current practice with Diff-Quik II is to examine all 
positives and many of the apparent negatives with a 100x oil immersion lens. I 
think this really makes a difference, particularly if your high-dry (45x) 
objective is less than perfect.

Diff-Quik is of course a trade name. My personal experience is that generics 
work quite as well.

Bob Richmond
Samurai Pathologist
Knoxville TN
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