[Histonet] In-situ hybridization help please

LaFriniere, Mike michael_lafriniere <@t> memorial.org
Thu Dec 4 10:40:36 CST 2003


Before I can respond with the experience I have had with in-situ, may I ask
first, which system are you using?

Michael LaFriniere PA, HT(ASCP)
Pathology Manger
Memorial Hospital
Chattanooga TN

-----Original Message-----
From: Peter Munday [mailto:p.munday <@t> inpharmatica.co.uk] 
Sent: Thursday, December 04, 2003 6:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] In-situ hybridization help please

Hello Histonetters,

I am currently doing in-situ hybridization on paraffin embedded tissue array
sections.  Very often I get a signal in the nucleus, which I think is a
little odd as I would expect to see it in the cytoplasm.  
Has anybody else encounted this?
If so, is it real?
If it isn't real, what can be done about it?


Peter Munday

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