[Histonet] Citrate Buffer pH

[Houston, Ronnie]

Patsy,
 
We routinely use Reveal and Borg, both from Biocare Medical, with great
success in  a B&D steamer, 20 minutes, followed by 2 rinses in hot buffer,
and 20 minute cool down. Very rarely use pressure cooker, and don't use
micrwave at all.
 
Ronnie
 
Ronnie Houston 
Regional Histology Operations Manager 
Bon Secours HealthPartners Laboratories 
5801 Bremo Road 
Richmond, VA 23226 
(804) 287 7972 
ronnie_houston <@t> bshsi.com 
 
-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> colobio.com]
Sent: Wednesday, December 03, 2003 1:19 PM
To: Jackie.O'Connor <@t> abbott.com; Pat Flannery
Cc: Histonet <@t> lists.utsouthwestern.edu;
histonet-admin <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Citrate Buffer pH


Has anybody tried the AR reagents that deparaffinzed such as BioCare
Decloaker in a steamer in place of the pressure cooker?  PC is too harsh for
some of my stuff but I like using these retrieval/deparaffination reagents.
Patsy
 
-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of
Jackie.O'Connor <@t> abbott.com
Sent: Tuesday, December 02, 2003 2:50 PM
To: Pat Flannery
Cc: Histonet <@t> lists.utsouthwestern.edu;
histonet-admin <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Citrate Buffer pH
 

I've been using a product from Biocare, a Universal Decloaker (6.0 citrate
buffer) , which deparaffinizes during HIER in their pressure cooker
(Decloaking Chamber) - they are a very Trekkie oriented company.  It works
great - saves a bunch o' steps.  You might try contacting them for the
specifics.  I don't think about the nature of these things if they work - I
just follow along blindly . . . . . . . . .sometimes. 

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics




 
Pat Flannery <pjfnefro <@t> duke.edu> 
Sent by: histonet-admin <@t> lists.utsouthwestern.edu 
12/02/2003 03:00 PM 
        
        To:        Histonet <@t> lists.utsouthwestern.edu 
        cc:         
        Subject:        [Histonet] Citrate Buffer pH



I have a procedure for deparaffinizing (their word, not mine) tissue 
sections in order to do immunostaining using the Vector MOM kit.  It 
calls for heating the section (briefly) in a microwave in 0.1M Citrate 
Buffer.  The procedure  does not specify the pH of the buffer, just to 
make it by mixing 0.1M NaCitrate and 0.1M citric acid.  Does this sound 
familiar to anyone out there?

-- 

-Patrick J. (Pat) Flannery
Division of Nephrology (that's kidneys to you)
Box 3014 (that's NOT "PO" just "Box")
Duke University Medical Center
Durham, NC  27710
E-mail: pjfnefro <@t> duke.edu (preferred)
        FLANN002 <@t> MC.DUKE.EDU (also works)
Voice:   (919)660-6863
Fax:     (919)684-2929




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