[Histonet] HIER using B & D rice steamer

[Sebree Linda A.]

One of our pathologists is looking for an inexpensive way to antigen retrieve tissues for staining with CD3, CD68 and CD56.  I suggested a Black and Decker rice steamer although I personally have no experience with one.  Is anyone out there willing to share their retrieval protocol?  I (and he) would greatly appreciate it.

Thank you,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174