[Histonet] Paraffin embedding following storage in 70% alcohol

[John Kiernan]

Of course you are right!
This is yet another example of an error in a procedure informally handed on from person to person! Always work from a book. Even a very old one will be OK for paraffin embedding.
John Kiernan.
https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html
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________________________________
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
Sent: November 12, 2021 11:03 AM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Paraffin embedding following storage in 70% alcohol

I am working with a grad student on a project dealing with equine articular
cartilage.

The protocol she sent me for embedding the tissue samples goes directly
from 70% alcohol to the embedding step in paraffin.


Correct me if I am wrong but shouldn't the tissue be dehydrated fully and
cleared before embedding the samples?
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