[Histonet] Paraffin embedding following storage in 70% alcohol

Charles Riley criley at udel.edu
Fri Nov 12 10:03:51 CST 2021


I am working with a grad student on a project dealing with equine articular
cartilage.

The protocol she sent me for embedding the tissue samples goes directly
from 70% alcohol to the embedding step in paraffin.


Correct me if I am wrong but shouldn't the tissue be dehydrated fully and
cleared before embedding the samples?


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