[Histonet] Re: Reference to microtome micrometer thickness

Fri Nov 28 03:47:42 CST 2014

Hi Maxim,

Does measuring section thickness on slide scanners get affected by the surface imperfections on the section?

It has been indicated to me that surface imperfections certainly hinder scanning speed but what about the actual thickness measurements?

Kind regards

Richard Vernon
Strategic Product Manager - Sectioning Products
Anatomical Pathology Division

Thermo Fisher Scientific
Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, UK
Tel:+44 (0) 1928 534122 | Mobile:+44 (0) 7825 119070
richard.vernon <@t> thermofisher.com | http//:www.thermoscientific.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: 28 November 2014 09:43
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 132, Issue 29

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Today's Topics:

   1. Re: Reference to microtome micrometer thickness (Barry Rittman)
   2. Congo Red problems (Wheelock, Timothy R.)
   3. Re: Congo Red problems (Carlos Defeo)
   4. RE: Formalin smell in the last paraffin station in vip	6
      tissue tek (Jamal)

----------------------------------------------------------------------

Message: 1
Date: Wed, 26 Nov 2014 12:05:53 -0600
From: Barry Rittman <barryrittman <@t> gmail.com>
Subject: Re: [Histonet] Reference to microtome micrometer thickness
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<CA+0tsF3q_YxA2y2bjRgxeF8tiX_FAw6Miv-BnaYME75X7-hULg <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

It has been a while since I read articles regarding section thickness but I have some comments:
1.

On Sat, Nov 22, 2014 at 8:37 PM, Maxim Peshkov <Maxim_71 <@t> mail.ru> wrote:

> Reuel,
> It is right, that there are many external reasons for a thickness of 
> paraffin sections.
> It is possible to measure the final stained sections with mathematical 
> methods by slide scanner.
> I hope that the slide-scanner can be easily to calculate thickness of 
> section by their soft.
> Each individually sections will have own specific thickness.
>
> Sincerely,
> Maxim Peshkov,
> Russia,
> Taganrog.
>                        mailto:Maxim_71 <@t> mail.ru
>
>
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> Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 2
Date: Wed, 26 Nov 2014 20:48:00 +0000
From: "Wheelock, Timothy R." <twheelock <@t> mclean.harvard.edu>
Subject: [Histonet] Congo Red problems
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<69718C0B0B3C414D9F8E7214AD400CC9773C9601 <@t> PHSX10MB11.partners.org>
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Hi All:

Lately, I have been having problems with my Congo Red staining too lightly.
The Bielschowsky silver stain shows lots of amyloid in the cerebral blood vessels and the cores of the senile plaques.
However,  the Congo Red stain is pretty faint, although you can still see it.
I have just bought a new bottle of Congo red from Sigma, in case it was the particular batch of dye.
My protocol, which I have always used, is as follows:

1. Deparaffinize sections and bring down to 95% ethanol 2. Stain in 1% Congo Red solution for 10 minutes.
3. Differentiate in Potassium Hydroxide solution; 3 dips 4. Rinse in 4-5 changes of tap water.
5. Counter-stain in Harris Hematoxylin for 1 minute.
6. Differentiate in Acid Alcohol; 10 dips or so.
7. Blue Hematoxylin in running tap water for 10 minutes.
8. 95% ethanol for only 3 dips, then right into absolute ethanol.
9. 20 dips in first absolute ethanol
10. 1 minute in absolute ethanol
11. Clear and mount.

Any suggestions would be really appreciated,

Tim Wheelock
Harvard Brain Bank
Mclean Hospital
Belmont, MA

The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.

------------------------------

Message: 3
Date: Wed, 26 Nov 2014 21:28:02 -0200
From: "Carlos Defeo" <latecor <@t> adinet.com.uy>
Subject: Re: [Histonet] Congo Red problems
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Cc: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <201411262128020715.000E90C2 <@t> adinet.com.uy>
Content-Type: text/plain; charset="us-ascii"

Why don?t you use the classic Highman?s Congo red hidroalcoholic solution? (0,5% in 80% ethanol).
Best luck,
Carlos.

*********** REPLY SEPARATOR  ***********

On 26/11/2014 at 20:48 Wheelock, Timothy R. wrote:

>Hi All:
>
>Lately, I have been having problems with my Congo Red staining too lightly.
>The Bielschowsky silver stain shows lots of amyloid in the cerebral 
>blood vessels and the cores of the senile plaques.
>However,  the Congo Red stain is pretty faint, although you can still 
>see it.
>I have just bought a new bottle of Congo red from Sigma, in case it was 
>the particular batch of dye.
>My protocol, which I have always used, is as follows:
>
>1. Deparaffinize sections and bring down to 95% ethanol 2. Stain in 1% 
>Congo Red solution for 10 minutes.
>3. Differentiate in Potassium Hydroxide solution; 3 dips 4. Rinse in 
>4-5 changes of tap water.
>5. Counter-stain in Harris Hematoxylin for 1 minute.
>6. Differentiate in Acid Alcohol; 10 dips or so.
>7. Blue Hematoxylin in running tap water for 10 minutes.
>8. 95% ethanol for only 3 dips, then right into absolute ethanol.
>9. 20 dips in first absolute ethanol
>10. 1 minute in absolute ethanol
>11. Clear and mount.
>
>Any suggestions would be really appreciated,
>
>Tim Wheelock
>Harvard Brain Bank
>Mclean Hospital
>Belmont, MA
>
>
>The information in this e-mail is intended only for the person to whom 
>it is addressed. If you believe this e-mail was sent to you in error 
>and the e-mail contains patient information, please contact the 
>Partners Compliance HelpLine at http://www.partners.org/complianceline 
>. If the e-mail was sent to you in error but does not contain patient 
>information, please contact the sender and properly dispose of the 
>e-mail.
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 4
Date: Tue, 25 Nov 2014 11:13:09 +0300
From: "Jamal" <j.rowaihi <@t> alborglaboratories.com>
Subject: RE: [Histonet] Formalin smell in the last paraffin station in
	vip	6	tissue tek
To: "'Arun Jyothi S.P'" <arunjyothisp <@t> gmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<00dd01d00887$a8161110$f8423330$@rowaihi <@t> alborglaboratories.com>
Content-Type: text/plain;	charset="UTF-8"

Hi
If the paraffin are overheated it gives abnormal odor.
Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point.
Please update me.

Best Regards,

Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg Medical Laboratories |  Mobile +966 503629832| j.rowaihi <@t> alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    | Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     | www.alborglaboratories.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P
Sent: Tuesday, November 25, 2014 9:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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End of Histonet Digest, Vol 132, Issue 29
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