[Histonet] Congo Red problems

[Carlos Defeo]

Why don´t you use the classic Highman´s Congo red hidroalcoholic solution? (0,5% in 80% ethanol).
Best luck,
Carlos.


*********** REPLY SEPARATOR  ***********

On 26/11/2014 at 20:48 Wheelock, Timothy R. wrote:

>Hi All:
>
>Lately, I have been having problems with my Congo Red staining too lightly.
>The Bielschowsky silver stain shows lots of amyloid in the cerebral blood
>vessels and the cores of the senile plaques.
>However,  the Congo Red stain is pretty faint, although you can still see
>it.
>I have just bought a new bottle of Congo red from Sigma, in case it was
>the particular batch of dye.
>My protocol, which I have always used, is as follows:
>
>1. Deparaffinize sections and bring down to 95% ethanol
>2. Stain in 1% Congo Red solution for 10 minutes.
>3. Differentiate in Potassium Hydroxide solution; 3 dips
>4. Rinse in 4-5 changes of tap water.
>5. Counter-stain in Harris Hematoxylin for 1 minute.
>6. Differentiate in Acid Alcohol; 10 dips or so.
>7. Blue Hematoxylin in running tap water for 10 minutes.
>8. 95% ethanol for only 3 dips, then right into absolute ethanol.
>9. 20 dips in first absolute ethanol
>10. 1 minute in absolute ethanol
>11. Clear and mount.
>
>Any suggestions would be really appreciated,
>
>Tim Wheelock
>Harvard Brain Bank
>Mclean Hospital
>Belmont, MA
>
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